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AptoMIA

Protein-aptamer binding technique

We have two types of protein-aptamer binding techniques that bind aptamers highly specifically to target protein.

Method 1. Protein capture with aptamer pairs

  • Simultaneous detection of multiple biomarkers of interest in serum or plasma samples using aptamer pairs
  • Enhancement of signal-to-noise ratio by sandwich type assay
  • Patented platform technology for aptamer pair screening (Aptamer sandwich assays, US8785132B2)

1

Bead-capture aptamer binding

2

Formation of capture aptamer-biomarker-detection aptamer complex

3

Elution of detection aptamer

Method 2. Protein capture with single aptamer

  • Simultaneous capture of multiple biomarkers with single aptamers that can specifically bind to their target.
  • Increase of specificity by repeated washing of the aptamer-protein complex
  • Applied when the analysis of a large number of different biomarkers in a single well.

1

Aptamer-protein binding

2

Immobilization of aptamer-protein complex on SA bead & Biotinylation of protein

3

Photocleavage of the aptamer-protein complex from the bead & Immobilization of the complex on the second bead

4

Elution of detection aptamer

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