As aptamers are oligonucleotide molecules, they can be applied to PCR amplification and hybridization methods. Therefore, the concentration of biomarkers can be indirectly quantified by measuring the concentration of target-bound aptamers.
Our company possesses two quantitation techniques; bead microarray and multiplex PCR. Depending on the number of biomarkers that need to be detected, the quantitation technique can be selected.
Number of simultaneous measurements
Up to 100 targets
< 5 targets
Coefficient of variation
Multivariate index assay of 5 or more targets
Multivariate index assay of 5 or fewer targets
Method 1. Bead Microarray
Luminex bead-based aptamer quantitation technology using hybridization method
Protein quantitation by the formation of primer-aptamer-primer(biotinylated) structure.
Multiplexed microsphere-based suspension array platform capable of analyzing up to 100 different targets in a single reaction vessel.
Suitable for measuring markers of 5 or more
Method 2. Multiplex PCR
Multiplex PCR-based aptamer quantitation technology using primers and probes for selective amplification
Excellent detection limits down to 10-18M, time-saving, and cost-effective